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Gallic acid standard curve

Over 80% New & Buy It Now; This is the New eBay. Find Acid now! Looking For Acid? Find It All On eBay with Fast and Free Shipping It's a diagram in which is detailed the procedure to prepare a standard curve of gallic acid in a concentration range between 0 - 500 mg/L and I'd like you to check the following and tell me if. I need just how to make serial dilution for six serial dilution for Gallic acid standard phenol 0.5m ( stock solution)g down dilution to .05mg/ml to do standard curve Cite 16th Jan, 201 Galic Acid Standard INTRODUCTION. Gallic acid is a polyphenol produced by plants, which shows antioxidant properties. It is commonly used to measure polyphenol concentration but could also be used to measure antioxidant capacity. It is present in a wide variety of plant-based food samples. Prepare the calibration curves in 1.5 ml tubes as.

Gallic acid (also known as 3,4,5-trihydroxybenzoic acid) is a trihydroxybenzoic acid with the formula C 6 H 2 (OH) 3 CO 2 H. It is classified as a phenolic acid. It is found in gallnuts, sumac, witch hazel, tea leaves, oak bark, and other plants. It is a white solid, although samples are typically brown owing to partial oxidation The exact reasons of choosing Gallic acid as a standard are: -Partly for historical reasons, -Inexpensive, -Soluble in water, -Recrystallized easily from water, -Readily dried, and -Stable in the dry form. for these reasons Gallic acid is #1, but catechin can also be used as standard in Folin-Ciocalteu assay A standard gallic acid curve was constructed by preparing the dilutions of (0.1, 0.5, 1.0, 2.5 and 5 mg/ml) in methanol from standard 1 solution of gallic acid. 100 µl of each of these dilutions were mixed with 500 µl of water and then with 100 µl of Folin-Ciocalteu reagent and allowed t

We have also tested the stability of gallic acid standard solutions and we can say they lose less than 5% of their value over two weeks when refrigerated and kept tightly closed. You MUST make up your own standard curve each time the analysis is run. Do not use this data as a standard curve Gallic acid was used as standard. The calibration curve was plotted using standard gallic acid. The data for total phenolic contents of polyherbal formulation were expressed as mg of gallic acid equivalent weight (GAE)/ 100 g of dry mass (Bhalodia et al., 2011; Patel et al., 2010

reagent. Gallic acid was used as a standard compound and the total phenols were expressed as mg/g gallic acid equivalent using the standard standard curve equation: y = 0.006x + 0.038, R2= 0.999, Where y is absorbance at 760 nm and x is total phenolic content in the extracts of C longa, C. amada & C. caecia expressed in mg/gm Gallic acid was used as a standard compound and the total phenols were expressed as mg / g gallic acid equivalents (standard curve equation: y= 1.387x +0.055, R2=0.903). The total phenol varied from 8.29±0.52 to 32.54±0.35 mg GAE /g. The highest concentration of phenols was measured in methanol and aqueou Standard Curve Gallic Acid. 39 c. Escherichia coli . d. Salmonella thypimurium . e. Listeria monocytogenes . Appendix 3. Output of One Way ANOVA (Post Hoc Test) between Harvesting Time on Total Phenolic Compound of Z.officinale Roscoe crude extracts. 40 Appendix 4. Output of independent sample T-test between fresh and powdered ginger on.

Standard curve for gallic acid. The coefficient of determination (R2) of the standard curve was 0.99 The total phenolic content was calculated with the help of the graph shown in Figure 1, and the standard curve equation was y = 0.007x + 0.186, where R 2 = 0.992. The total phenolic contents (Gallic acid equivalents, mg/g) in the ethanolic and aqueous extracts were calculated to be 326.28 and 319.14 mg/g, respectively 1Each value is the average of three measurements ± standard deviation. The total phenolic contents in the examined plant extracts using the Folin-Ciocalteu's reagent is expressed in terms of gallic acid equivalent (the standard curve equation: y = 7.026x - 0.0191, r2 = 0.999). The values obtained for the concentration of total phenols ar

Gallic acid standard calibration curve for the quantification of total phenolic content. Using the equation obtained from calibration curve, the ethanol extract (CDA Et) showed the highest phenolic content (47.8 mM GAE) followed by acetone (38.6 mM GAE) and n -hexane (27.2 mM GAE) while chloroform and dichloromethane extracts showed the lowest. Array, USA) and the results were expressed in gallic acid equivalents (GAE; mg/100g fresh mass) using a gallic acid (0-.1mg/mL) standard curve. Additional dilution was done if the absorbance value measured was over the linear range of the standard curve. Total carotenoids content was determined by th Different concentration of gallic acid was prepared to plot the standard curve for phytoextracts concentration determination. The stock solution was prepared by dissolving 100 mg of gallic acid in 100 mL ethanol. The concentrations prepared through dilution were 0, 1, 2.5 5.0, 10, 25, 50, 100, 200 and 300 µg/mL of gallic acid 0.353 for tannic acid. Results of statistical analysis shows that present HPLC method for determination of gallic acid and tannic acid is simple, precise, accurate and suitable for routine analysis of gallic acid and tannic acid in BV. The developed fingerprints can be used as a standard and gallic acid and tannic acid can be used as a possibl Graph 1 Calibration curve for Standard Gallic acid A standard curve of gallic acid was constructed to determine the amount of total phenolic contents in the black grams. The equation for the gallic acid was expressed as y= 0.002x (R2=0.973). The results of the study were expressed as gallic acid equivalent (GAE) in μg/ g of dry weight

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How to build Gallic Acid Curve and calculate Total

  1. Generally, gallic acid is used as the reference standard compound and results are expressed as gallic acid equivalents (mg/mL). The assay has been used as a measure of total phenolics in natural products, but the Standard curve example for microplate assay procedure is shown: RELATED PRODUCTS
  2. Gallic Acid standard including blank as described in Assay Protocol. The average AUC is 2.2 for blank and 7.0 for sample. Net AUC = AUC (Antioxidant) - AUC (blank) = 7.0 - 2.2 = 4.8 Based on the Gallic Acid antioxidant standard curve, the equivalent Gallic Acid concentration is 400 µM, therefore
  3. Ciocalteu method, are reported as gallic acid equivalents by reference to standard curve (y = 0.0063x, r2 = 0.987). The total phenolic contents were usually higher in peels with the range of 104.2-223.2 respect to tissues with the range of 66.5-396.8 mg gallic acid equivalent/g of extract powder. C. reticulata var. Clementine and C. reticulat
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  5. GALLIC ACID STANDARD ST08003 INTRODUCTION Gallic acid is a polyphenol produced by plants, which shows antioxidant properties. It is calibration curves in 1.5 ml tubes as shown below: Standard (µl) Diluent (µl) TEAC (µM) 1 0 1000 0 2 10 990 10 3 25 975 25 4 50 950 50 5 75 925.
  6. Regression equation was calculated in the form Y = aX + b. In the formula, X and Y are concentration of standard solution (mg/mL) and corresponding absorbance. a and b correspond to slope and intercept, respectively. A calibration curve of gallic acid (ranging from 1 to 10 μg/ml) was prepared and listed in Figure 1

Can anyone help how can I make the standard curve for

The standard curve of Gallic acid for TPC. Figure S2. The standard curve of Quercetin for TFC. Figure S3. The standard curve of Catechin for TCTC. y = 9.005x + 0.008 The standard curve of Tannin acid for TTC. Figure S5. The standard curve of Trolox for DPPH. Figure S6. The standard curve of Trolox for FRAP. y = 0.7305x + 0.016 Gallic acid (0.05-0.5 mg/ml) is often used to produce standard calibration curve and the total phenolic content expressed as mg equivalent of gallic acid (mg GAE) per gram dry weight of the extract by computing with standard calibration curve. Fo

Galic Acid Standard, for eBQC calibration ST0800

Standard curve of gallic acid. 3.5. Reproducibility Test Six pieces of P. chinense (2.0 g) was treated by water bath for extraction of gallic acid under the designed con-ditions. The experiment was repeated six times, and measured 6 times according to the method of 2.1, = RSD 1.34%. It showed that the repeatability of the method was good. 3.6 Gallic acid is chosen as a reference standard, and the activity of sample is expressed as gallic acid equivalents. The method is rigorously validated through linearity, precision, accuracy, and ruggedness Gallic acid has been used as a reference standard in the high performance liquid chromatography coupled triple quadrupole mass spectrometric (HPLC/QqQ MS) analysis of gallic acid in rat medicated plural serum containing Guizhi Fuling Wan, a chinese herbal formula used for the treatment of ovarian cancer

a Standard curve of gallic acid b Standard curve of

Gallic acid - Wikipedi

The determination of gallic acid (GA) content by a graphene modified glassy carbon electrode (graphene/GCE) was studied. The electrochemical behavior of GA on this electrode was studied by cyclic voltammetry (CV), and the standard curve was constructed by differential pulse voltammetry (DPV) Standard ellagic acid (Rf:0.47) and gallic acid (Rf:0.56) showed single peaks in HPTLC chromatogram (Fig. 2 and 3). Calibration curve of ellagic acid was prepared by plotting concentrations of ellagic acid versus average area of the peak. Similarly, the calibration curve of gallic acid was prepared (Fig. 4 and 5). The formulation samples were.

How do we choose the standard for calibration curves

The densitometric chromatogram of HPTLC fingerprint of the alcoholic extract, isolate, and standard gallic acid was obtained. The calibration curve of gallic acid was linear over a concentration range (0.2- 2 microg/ml) with a good correlation coefficient (R 2 = 0.9986) and coefficient of variation as CV- 2.4663%. The method was validated for. The column was maintained at room temperature, and the samples were kept at 15°C and then analyzed with the Empower pro Software. The standard yield curve of pyrogallol was y = 1318523.5x+6474.1, correlation coefficient R = 0.99951; the standard curve of gallic acid was y = 13379425.0x+60043.8, correlation coefficient R = 0.99951

Gallic acid (also known as 3,4,5-trihydroxybenzoic acid) is a trihydroxybenzoic acid with the formula C6H2(OH)3CO2H. It is classified as a phenolic acid. It is found in gallnuts, sumac, witch hazel, tea leaves, oak bark, and other plants. It is a white solid, although samples are typically brown owing to partial oxidation. Salts and esters of gallic acid are termed gallates standard curve of gallic acid as shown in Graph No. 1. Data of calibration curve for gallic acid are shown in Table No. 1. Total amount of phenolic compounds in Amaranthus gangeticus were done in triplicates and concentrations of total phenolic content in various extracts were determined and expressed as Gallic acid in mg equivalents per gram.

curve, which is usually represented as the area under the curve (AUC). The AUC is used to quantify the total hydroxyl radical antioxidant activity in a sample and is compared to a gallic acid antioxidant standard curve. Cell Biolabs' OxiSelect™ HORAC Activity Assay is a fast and reliable kit for the direct measuremen Absorption spectra (400 to 900 nm) of reference compounds (gallic acid, tannic acid, catechin and pyrogallol) and CE from L. brasiliense by Folin-Ciocalteu reaction after 30 min. Molecules 2013, 18 6855 calculations for curve data for the standard used in the validation runs, as well as the precision an Standard Gallic Acid Test Lab Report 1875 Words | 8 Pages. RESULTS AND DISCUSSION: Graph 1 Calibration curve for Standard Gallic acid A standard curve of gallic acid was constructed to determine the amount of total phenolic contents in the black grams. The equation for the gallic acid was expressed as y= 0.002x (R2=0.973) Folin Ciocalteu (F-C) assay is the most widely used and convenient method to determine the total phenolics content in foods, herbs, and other plant extracts. Different phenolics standards such as gallic acid, ferulic acid, chlorogenic acid, catechol, and vanillic acid have been used for calibration curves in this assay method. Comparison of these standards, in single or combination of two or.

Standard curve for Gallic acid (for TPC) (A) and quercetin

ensure the readings are within the standard curve range. 2. Standard Curve Preparation: Dilute the 100 mM Catechin Standard solution at a 1:100 ratio by adding 10 μl of the Standard solution to 990 μl of 70% ethanol (made from 200 proof ethanol and ddH 2 O) to obtain a 1 mM Catechin Standard solution. Add 0, 2, 4, 6, 8 an determined using the gallic acid and kaempferol calibration curves, respectively. RESULTS Linearity A calibration curve was established for gallic acid and kaempferol by injecting 4, 8, 12, 16, 20, and 24 µg/mL of standard solution. Linearity was tested by analyzing the average peak area of gallic acid and kaempfero secondary metabolites are expressed as Gallic Acid Equivalents (GAE mg/L) relative to the standard curve (refer to 'Data Preparation and Finalization' below). (viii) Standard Preparation Procedure • Prepare 5 mg/ml stock solution of Gallic Acid in 95% methanol solution in a 100ml volumetric flask every month measured at 765 nm. Different volumes of Gallic acid solution were used in same manner for calibration of standard curve and quantification was done in terms of mg equivalent of Gallic acid. The blank was prepared by using distilled water in place of sample/standard. B. Evaluation for Antioxidant Activity: i Standard curves obtained for gallic acid, caffeine, epicatechin and epigallocatechin gallate over the concentration range of 2.5-25 μg/ml. (Peak area ratio = Area under curve of the analyte/Area under curve of internal standard) Full size image

Standard curve was plotted with peak area as the ordinate and injection volume as the abscissa to obtain the regression equation: A=4963.95C-36.17, r=0.9997, which indicated good linearity within a gallic acid injection amount range of 0.02-0.41 μg Densitometric determination was carried out at specified wavelengths for different standard compounds in reflection/absorption mode. The calibration curves were linear in the range of 100‒600 ng per spot for salicylic acid, kaempferol, gallic acid, and protocatechuic acid Interaction between ascorbic acid and gallic acid in a model of compared with the standard curve of DMF. 2.4. Determination of protein carbonyl content The carbonyl group in glycated BSA was determined following a previously described method [24].Briefly, 400 μL of 10 mM DNP Briefly, different dilutions of gallic acid (0, 25, 50, 100, 250 and 500 μg/mL) were produced to generate a standard curve. From each urine sample, 25 μL was diluted with 975 μL of water. The standards and samples were incubated for 90 minutes at room temperature after the addition of 100 μL of 0.1% solution of FBBB and 400 μL of 5% sodium.

using gallic acid as a standard (Chaves et al., 2013). A sample was dissolved in water with 0.5ml of Folin-Ciocalteu solu-tion (Sigma-Aldrich)andleftatrestfor2min.Thenitwasaddedto 1 ml of sodium carbonate solution 20% (w/w) and was left to stand for 10min. The calibration curve was prepared with a gallic acid standard in different. The working standards were run in HPLC and used to develop the calibration curve. 2.8 Preparation of gallic acid standards. he standards used were gallic acid. 0.1 g of gallic acid monohydrate (M.W. 188.14) was weighed into 100 mL one-mark volumetric flask, dissolved in water and diluted to the mark before mixing Gallic acid is an eco-friendly compound which can replace the currently available chemical pesticides which are toxic to plants and humans. Application of gallic acid isolated from cashewnut shell will be a cheap source green-pesticide for preventing R. solanacearum mediated wilting disease in tomato plants curcuminoids and gallic acid respectively. The standard calibration curve and standard table for curcuminoids and gallic acid is given in fig.1 and table.1 respectively. Hence the proposed method can be used for the routinely employed for the estimation of curcuminoids and gallic acid in bulk and pharmaceutical ayurvadic dosage forms. RESULT gallic acid containing in the semipurified fraction iso-lated from T. chebula galls and the pure gallic acid loaded in this developed elastic niosome was evaluated for topi-cal antiaging applications. Materials and methods Materials Standard gallic acid, Tween 61 (polyoxyethylene sorbitan monostearate), cholesterol, and Sephadex-G-50 wer

Folin-Ciocalteau Micro Method for Total Phenol in Wine

Component III was identified as gallic acid, with the high-purity of 96.4%, by comparative HPLC analysis with standard products. Microcalorimetry-based determination of the antibacterial effects of the isolated fractions The inhibitory effects of the isolated fractions on A. hydrophila B11 and A. veronii B5 Calibration curve: The calibration curve must be prepared using 100-500 µL aliquots of the tannic acid solution, 500 μL of the Folin-Ciocalteu solution and 1 mL of the sodium carbonate solution. The final volume should be adjusted to 10 mL with distilled water. The final tannic acid concentration will be 1-5 µg/mL

Standard curve for gallic acid

University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Masters Theses Graduate School 5-2016 High intensity ultrasound assisted extraction of oak compound The amount of total phenolic groups in CNTs was expressed as gallic acid equivalent concentration by using an equation obtained from a gallic acid calibration curve. This was recorded by employing five different gallic acid standard solutions in water Gallic acid is a widely available phenolic acid that has been shown to 87 possess strong antimicrobial activity (Chanwitheesuk, Teerawutgulrag, Kilburn, & 88 Rakariyatham, 2007). 141 values were tested to achieve a standard curve. Square film pieces (20 × 20 mm) wer

Spectrophotometric determination of the total phenolic

Gallic acid calibration curve (Abs vs Conc) for evaluationDifferential Cytotoxicity of Triphala and its Phenolic

Abstract. A validated rapid HPLC-PDA method was developed for identification and quantification of five tannin-related constituents gallic acid (GA), corilagin (CL), chebulagic acid (CB), ellagic acid (EA) and chebulinic acid (CN) in the extracts prepared from the bark and fruits of four Terminalia species available in India. The separation of the five analytes was achieved on an RP-18. The standard solution of gallic acid (100 μg/ml), quercetin (1000 μg/ml) and lupeol (100 μg/ml) in different volumes were located on the different TLC plate for preparation of calibration curve (1-6 μl of gallic acid, 2-6 μl of quercetin and 1-12 μl of lupeol) checked for reproducibility Calibration Curve of Gallic Acid was obtained by plotting peak area versus concentration of applied standard i.e. Gallic Acid (Table 1). Table 1 R f Range and AUC of standard gallic acid (Track 1-5), Alternanthera sessilis (Track 6) and Clerodendrum infortunatum (Track 7) S. no. Start position Maximum RF End position AUC Track 1 0.82 0.89 0.91. (4.6 × 250 mm, 5 μm) column was used for quantification and identification of Gallic acid. The HPLC chro-matograms for standard Gallic acid and methanolic extract of punica granatum peels using the developed method are shown in Figure 4 and Figure 5. The retention time for Gallic acid is 1.94 min ( ± 0.2 min)

Results: The calibration curve was linear over a concentration range of 0.05-5.0 μg/mL. The method was found to be accurate, precise and stable for gallic acid from spiked plasma at QC levels. The average method recoveries for gallic acid were over 85% and extraction recoveries between 78 and 87% The standard calibration curve for gallic acid is shown in Figure 5. The calibrated equation obtained from the gallic acid standard curve is y=38590x-240357 (R 2 =0.9992). Based on the peak areas on the chromatogram and the standard curve of gallic acid, the concentrations of gallic acid in ADSP and MESP are presented in Table 4 Standard solutions of gallic acid at concentrations of 0, 62.5, 125, and 150 mg/L were used to obtain a standard curve. These solutions were pipetted and diluted to 5 ml with distilled water. Then Folin- Ciocalteau reagent (0.5 ml) was added and the mixture was kept at room temperature for 2 min We developed a high-performance liquid chromatography-based method for simultaneous analysis of nine catechins, gallic acid, strictinin, caffeine, and theobromine in green tea by using catechol as an internal standard. Although the high cost and instability of the catechin reference standards limit the application of this method, the addition of ascorbic acid to the standard stock solution. The calibration curve was plotted using the absorbance of standard gallic acid against its concentration in the range between 0.2 and 0.0016 mg/mL. The TPC was calculated according to the standard curve equation of gallic acid (y = 13.463x + 0.0406, R 2 = 0.9991) and is expressed as mg gallic acid equivalents per 100 g dried sample (mg GAE/100.

in the diluted extract was derived from a standard curve of gallic acid ranging from 10-100 μg/ml and multiplied by the dilution factor to obtain µg/ml of the original extract. This value was then multiplied by total volume of extract and divided by weight of sample. This gallic acid equivalents value (GAE Standard marker Gallic acid (98% pure) was procured from Sigma Aldrich Chime (Steinheim, Germany). Silica gel 60F 254 (10x20 cm, 0.2 mm thick) HPTLC pre-coated plates were procured from Merck (Darmstadt, Germany). Preparation of standard solution A stock solution of Gallic acid (1000 μg /ml) was prepared b nm and 227 nm respectively and a calibration curve were plotted at these wavelength. A set of two simultaneous equations were established using the mean absorpitivity values of curcuminoids and gallic acid10. A 1=42.08 Cgallic acid + 22.13 Ccurcuminoids (i), A2=12.39 Cgallic acid + 62.37 Ccurcuminoids (ii). Where- 42.08 and 12.39 are th

Standard calibration curve of gallic acidThesis presentation of sumaiya nahid1: Glucose standard curve for reducing sugar estimationLinearity of the automated TAC assay A standard curve of 0

gallic acid equivalent by comparison with standard calibration curve. The samples were treated as above and also calculated total phenolic content as gallic acid. 5.2 Analysis of antioxidant inhibition power (Li et al., 2007). The clear filtrate from part 2 were pipetted 600 μL mixed with 600 μL of 0.1 mM DPPH the Standard curve of gallic acid was prepared by plotting absorbance versus concentration. The total phenolic content of ZBSE was determined using the standard calibration curve of gallic acid and the value was expressed as gallic acid equivalent (GAE)/100 g of dried plant material. 2.8 Iron Complexation Studies of Gallic Acid. Download. Iron Complexation Studies of Gallic Acid. Mohamed Taha. Ahmed Fazary. Mohamed Taha. Ahmed Fazary. Related Papers. Complex Formation Between Ferric(III), Chromium(III), and Cupric(II) Metal Ions and (O,N) and (O,O) Donor Ligands with Biological Relevance in Aqueous Solution To improve the antibacterial and antioxidant properties of chitosan (CS), CS grafted with gallic acid (GA) using recombinant bacterial laccase from Bacillus vallismortis fmb-103 (fmb-rL103) as a catalyst. The structures of grafted chitosans were identified using Fourier transform infrared spectroscopy (FT-IR) and UV visible spectrum (UV-Vis spectroscopy). After gallic acid grafting, the. the optimum range of concentration of 0.6-9.0 μg/mL for gallic acid, by the proposed method. The linear regression equation for gallic acid was y=0.079x-0.040, where x is the concentration of gallic acid in µg/mL, 0.040 is the intercept and y is the absorbance of the colored solution. LOD and LOQ were 0.0044 μg/mL and 0.0146 μg/mL respectively concentration of gallic acid standard solution were processed into the standard curve of gallic acid to form regression equation of the standard gallic acid solution. The sample solution was piped 100µL then followed by 500µL Folin-Ciocalteu (1:10 v/v water) addition prior incubation for 6min, 400µL Na 2 C

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